Unit of competency Outline
Date retreived
23/07/2026 6:23 PM AWST
23/07/2026 6:23 PM AWST
Whilst all efforts are made to provide accurate and timely information from the relevant source/documentation, please be aware that the information supplied may not be the most current version. The accuracy of the detail has not been confirmed by the Department and therefore should not be relied upon without first confirming the contents.
Perform histological tests
Perform histological tests
Unit of competency
National Code
MSL975057
MSL975057
State Code
OEX73
OEX73
TGA Status
Current
Current
DTWD Status
Approved
Approved
State Implementation and Classification
Approved Date
24/05/2023
Field of Education
019909 - Laboratory Technology
Original Release Date
24/05/2023
Nominal Hours
120
Description
This unit of competency describes the skills and knowledge to perform tests and procedures associated with processing and staining tissues for examination of tissue structure and abnormalities by pathologists and scientists to assist with disease diagnosis. The unit covers tests and procedures that are associated with anatomical pathology and may involve the use of automated processors and staining machines. This unit applies to laboratory technicians and technical officers in the biomedical sector. The unit principally refers to techniques performed on human tissues, but many aspects may be relevant to animal and plant tissues. This unit of competency assumes that the technical officer would perform tests and procedures under the close supervision of scientific and/or medical staff. Although a supervisor may not always be present, the technician will follow workplace procedures that will clearly describe the scope of permitted practice in modifying testing procedures, quality control procedures, interpretation of data, and for communicating test results to people outside the laboratory. Technical workers may need to interrupt their routine work in order to assist with or perform procedures to facilitate rapid diagnosis of specimens from patients in the operating theatre. The involvement of the technical officer in mortuary work will be determined by the workplace. Work of this nature will always be closely supervised by scientific/medical staff.No licensing or certification requirements exist at the time of publication. However, regulations and/or external accreditation requirements for laboratory operations exist, so local requirements should be checked. Relevant legislation, industry standards and codes of practice within Australia must also be applied.
Notes
Elements and Performance Criteria
1. Receive and check specimens and associated request forms
- 1.1 Identify specimens and request forms that do not comply with workplace requirements
- 1.2 Record any discrepancies and indicate action required
- 1.3 Register samples in accordance with workplace procedures, recording details that allow accurate tracking and chain of custody traceable to employee
- 1.4 Prepare work area for safe and effective workflows
2. Process tissue
- 2.1 Perform pre-use checks to ensure processor is fit for purpose
- 2.2 Inspect processor reagents for deterioration and adequate volume, and report any items requiring replacement
- 2.3 Record any issues that may impact on sample integrity and indicate action required
- 2.4 Check reagents are in sequential order
- 2.5 Select processor program for routine and non-routine samples
3. Embed tissue
- 3.1 Perform pre-use check to ensure embedding centre is fit for purpose and uninterrupted embedding of processor load
- 3.2 Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy
- 3.3 Apply procedures to prevent cross-contamination between tissues
- 3.4 Inspect blocks, reject and re-embed items that do not meet quality control standards
4. Cut tissue sections
- 4.1 Label required number of microscope slides in accordance with workplace traceability requirements
- 4.2 Check the flotation bath is ready and satisfactory for use
- 4.3 Prepare and adjust all external parts of a microtome and associated equipment for routine sample processing
- 4.4 Secure block in microtome following specified safety directions
- 4.5 Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts
- 4.6 Cut representative sections at the required thickness according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing
- 4.7 Float sections onto water bath to flatten tissues and pick up sections onto microscope slides ensuring patient identification on slides matches that on the block
- 4.8 Compare the blocks and sections ensuring the tissue on slides matches that in the blocks
- 4.9 Apply procedures to prevent cross-contamination between patient tissues
- 4.10 Identify, troubleshoot and resolve common section quality control issues
- 4.11 Inspect sections and reject any that do not meet quality control standards
5. Stain tissue sections
- 5.1 Prepare any labile reagents for immediate use
- 5.2 Select reagents for specified technique, ensuring reagent sequence matches workplace procedure
- 5.3 Stain sections in accordance with workplace procedures using the required quality control section and accommodating any authorised variations
- 5.4 Mount slides using medium compatible with staining technique ensuring no bubbles
- 5.5 Examine control sections microscopically to ensure expected staining outcomes are achieved
- 5.6 Confirm microscopically that the type/disease is appropriate and still present in the control sections for each stain
- 5.7 Label specimens in accordance with workplace traceability requirements
- 5.8 Collate, approve and release slides to pathologist in accordance with workplace procedures
6. Maintain laboratory records
- 6.1 Complete control stain quality assessment forms to report quality control outcome
- 6.2 File and store samples to facilitate efficient retrieval as required
- 6.3 Maintain instrument logs as required by accreditation checks
No information
No information
Replaces
| State Code | National Code | Title | Type |
|---|---|---|---|
| OAC58 | MSL975029 | Perform histological tests | Unit of competency |
| State Code | National Code | Title | Type |
|---|---|---|---|
| AF144 | MSLSS00003 | Histotechnology Skill Set | Skill set |
| BIX6 | MSL40122 | Certificate IV in Laboratory Techniques | Qualification |
| BIX5 | MSL50122 | Diploma of Laboratory Technology | Qualification |